Patrick Joseph Buckley, M.D., Ph.D.

Professor of Pathology

Department:
Pathology

Division:
Pathology Clinical Services

Email:
patrickbuckleydukeedu

Mailing Address:
Box 3712, DUMC
Durham,, NC 27710

Telephone:
919-681-6878

Training:
M.D., Washington University School of MedicinePh.D., University of Pennsylvania

Last Updated:
December 19, 2006

Clinical Interests:
Hematopathology and flow cytometry including bone marrow and lymph nodeinterpretation for adult and pediatric hematopoietic diseases; 27 yearsexperience in adult and pediatric hematologic diseases.Please send all consultation slides to: Patrick J. Buckley, M.D., Ph.D., c/oCarol Tate, Consultation Services, Dept. Pathology, DUMC 3712, Room M255,Davison Bldg, Green Zone, Erwin Rd, Durham, NC 27710

Research Interests:


1. The use of morphology, flow cytometry, cytogenetics, molecular techniques, and analysis of cell products to study the diagnosis, classification, and biological features of lymphoid and myeloid neoplasms (lymphoma/leukemia) in adults and children.  

2. Investigation of new strategies for educating residents for careers in anatomic pathology and laboratory medicine.

Publications:
2006 -- Gong JZ, Bayerl MG, Sandhaus LM, Sebastian S, Rehder C, Routbort M, Lagoo AS, Szabolcs P, Chiu J, Comito, Bucklry PJ: Post-transplant lymphoproliferative disorder after umbilical cord blood transplantation in children (Am J Surgical Pathol, 30:328-336 2006).

2006 -- Lagoo AS, Gong JZ, Stenzel TT, Goodman B, Buckley PJ, Chao NJ, Gasparetto C, Vredenburgh JJ, Long GD, Rizzieri DA: Significance of morphologically detectable disease and donor engraftment studies early post-transplant bone marrow after non-myeloablative strm cell transplant (Archives of Pathology and Laboratory Medicine 130:1479-88,2006).

2005 -- Talbot J, Ibom VK, Barrier R, Niedzwieki D, DeCastro C, Moore JO, Buckley P, Laney R,Stevenson D, Brumbaugh, H, Gockerman J: Dose-intense cyclophosphamide and etoposide for patients with refractory or high-risk non-Hodgkin lymphoma. Clinical Lymphoma 5:116-122, 2004.



2004 -- Pubmed # 15236260 -- Gong JZ, Snyder MJ, Lagoo AS, Vollmer RT, Dash RR, Madden JF, Buckley PJ, Jones CK. Diagnostic impact of core-needle biopsy on fine-needle aspiration of non-Hodgkin lymphoma.  Diagn Cytopathol.  2004 Jul;31(1):23-30.

2004 -- Safley AA, Buckley PJ, Creager A, Dash R, Dodd L, Goodman BK, Jones CK, Lagoo AS, Stenzel TT, Wang W, Xie B, Gong JZ: Primary diadnosis of non-Hodgkin lymphoma by fine needle aspiration: A combined cytologic, flow cytometric, and molecular approach. Arch. Pathol. Lab. Med. 128: 1395-1403, 2004.

2003 -- Pubmed # 12885460 -- Tirado CA, Golembiewski-Ruiz V, Horvatinovich J, Moore JO, Buckley PJ, Stenzel TT, Goodman BK. Cytogenetic and molecular analysis of an unusual case of acute promyelocytic leukemia with a t(15;17;17)(q22;q23;q21). Cancer Genet Cytogenet. 2003 Aug;145(1):31-7.

2003 -- Talbot J, Rizzieri DA, DeCastro CM, Moore JO, Buckley P, Laney R, Stevenson D, Brumbaugh H, Gockerman JP:High-dose cyclophosphamide and etoposide for patients with refractory acute myeloid leukemia: a case series.Am J Hematol. 73:295-6, 2003.



2001 -- Pubmed # 11764079 -- Gong JZ, Lagoo AS, Peters D, Horvatinovich J, Benz P, Buckley PJ. Value of CD23 determination by flow cytometry in differentiating mantle cell lymphoma from chronic lymphocytic leukemia/small lymphocytic lymphoma. Am J Clin Pathol. 2001 Dec;116(6):893-7.

2000 -- Pubmed # 11014641 -- Lagoo AS, Buckley PJ, Burchell LJ, Peters D, Fechner JH, Tsuchida M, Dong Y, Hong X, Brunner KG, Oberley TD, Hamawy MM, Knechtle SJ. Increased glomerular deposits of von Willebrand factor in chronic, but not acute, rejection of primate renal allografts. Transplantation. 2000 Sep 27;70(6):877-86.

1999 -- Friedl A, Stoesz SP, Buckley P, Gould MN: Neutrophil gelatinase-associated lipocalin in normal and neoplastic tissues-cell type-specific pattern of expression. Histochem J 31: 433-441, 1999.

1998 -- Armstrong N, Buckley P, Oberley T, Fechner J, Dong Y, Hong X, Kirk A, Neville D, Knechtle S: Analysis of primate renal allografts after T-cell depletion with anti-CD3-CRM9.  Transplantation 66:5-13, 1998.

1998 -- Knechtle ST, Fechner JH, Jr, Dong Y, Stravron S, Neville DM, Oberley T, Buckley P, Armstrong N, Rusterholz K, Hong X, Tsuchida M, Hamawy NM: Primate renal transplants using immunotoxin. Surgery 1334:438-447,1998.

1997 -- Farner N, Gan J, deJong JLO, Leary TP, Fenske TS, Buckley P, Dunlap S, Sondel PM:  Alteration of the CD34 and Tf-1 beta cell line profile in response to long term exposure to IL-1.  Cytokine 9:316-327, 1997.

1995 -- Korn E, Van Hoff J, Buckley P, Daughaday WH, Carpenter TO: Secretion of a Large Molecular-Weight Form of Insulin-like Growth Factor by a Primary Renal Tumor.  Med Pediat Oncol 24:329-396, 1995.

1993 -- Pubmed # 7691923 -- Tallini G, West AB, Buckley PJ. Diagnosis of gastrointestinal T-cell lymphomas in routinely processed tissues. J Clin Gastroenterol. 1993 Jul;17(1):57-66.

1993 -- Pubmed # 8470762 -- Hughes D, Buckley PJ. Idiopathic retroperitoneal fibrosis is a macrophage-rich process. Implications for its pathogenesis and treatment. Am J Surg Pathol. 1993 May;17(5):482-90.

1993 -- Pubmed # 8285532 -- Spinelli HM, Criscuolo GR, Tripps M, Buckley PJ. Massive orbital teratoma in the newborn. Ann Plast Surg. 1993 Nov;31(5):453-8.

1993 -- Schneider B, Touloukian R, Hajar F, Buckley PJ: Perianal angiocentric lymphoma:  Clinical confusion with Crohn's disease.  Digestive Disease and Science, 38:1162-1166, 1993.

1992 -- Pubmed # 1348582 -- Buckley PJ, O'Laughlin S, Komp DM. Histiocytes in familial and infection-induced/idiopathic hemophagocytic syndromes may exhibit phenotypic differences. Pediatr Pathol. 1992 Jan-Feb;12(1):51-66.

1992 -- Pubmed # 1327492 -- West AB, Buckley PJ. Mantle zone lymphoma in a gastric glomus tumor. Cancer. 1992 Nov 1;70(9):2246-9.

1992 -- O'Laughlin, S., Braverman, M., Smith-Jeffries M, Buckley, PJ:  Macrophages (Histiocytes) in various reactive and inflammatory conditions express different antigenic phenotypes.  Human Pathol 23: 1410-1418, 1992.

1992 -- Heald P, Buckley P, Gilliam A, Perez M, Knobler R, Kacinski B, Edelson R: Correlations of unique clinical, immunotypic and histologic findings in cutaneous gamma/delta T-cell lymphoma.  J Am Acad Dermatol 26:865-870, 1992.

1991 -- Pubmed # 2052920 -- Buckley PJ. Phenotypic subpopulations of macrophages and dendritic cells in human spleen. Scanning Microsc. 1991 Mar;5(1):147-57; discussion 157-8.

1991 -- Younes M, Podesta A, Helie M, Buckley PJ:  Infection of T- but not B-lymphocytes by cytomegalovirus in lymph node.  An immunophenotypic study.  Am J Surg Pathol 15(1): 75-80, 1991.

1991 -- Radin AT, Buckley PJ, Duffy TP.  Interferon therapy for agnogenic myeloid metaplasia complicated by immune hemolytic anemia.  Hematological Path 5:83-88, 1991.

1990 -- Pubmed # 2180014 -- Eisen RN, Buckley PJ, Rosai J. Immunophenotypic characterization of sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman disease).  Semin Diagn Pathol.  1990 Feb;7(1):74-82.

1990 -- Pubmed # 2173430 -- Sieber SC, Lopez V, Rosai J, Buckley PJ. Primary tumor of spleen with morphologic features of malignant fibrous histiocytoma. Immunohistochemical evidence for a macrophage origin. Am J Surg Pathol. 1990 Nov;14(11):1061-70.

1990 -- Havlik RJ, Touloukian RJ, Markowitz RI, Buckley PJ:  Partial splenectomy for splenic hamartoma.  J Ped Surg 25:1273-1275, 1990.

1989 -- Pubmed # 2642084 -- Komp DM, Buckley PJ, McNamara J, van Hoff J. Soluble interleukin-2 receptor in hemophagocytic histiocytoses: searching for markers of disease activity. Pediatr Hematol Oncol. 1989;6(3):253-64.

1989 -- Komp DM, McNamara J, and Buckley PJ: Elevated soluble interleukin-2 receptor in childhood hemophagocytic syndromes.  Blood 73:2128-2132, 1989.

1988 -- Pubmed # 3277357 -- Keller MS, Buckley PJ. Sonographic tissue pattern in neuroblastoma. AJR Am J Roentgenol. 1988 Mar;150(3):693.

1988 -- Goldstein J, Braverman BA, Salafia C, Buckley PJ:  The phenotype of human placental macrophages and its variation with gestational age.  Am. J. Pathol. 133:648-659, 1988.

1987 -- Pubmed # 3316374 -- Gerald WL, Sussman J, Buckley PJ. The immunologic approach to analysis of malignant lymphoma. Mantle zone lymphoma of the ileocecal region. J Clin Gastroenterol. 1987 Oct;9(5):588-98.

1987 -- Pubmed # 3307443 -- Buckley PJ, Smith MR, Braverman MF, Dickson SA. Human spleen contains phenotypic subsets of macrophages and dendritic cells that occupy discrete microanatomic locations. Am J Pathol. 1987 Sep;128(3):505-20.

1985 -- Pubmed # 2988860 -- Fitch S, Parvey LS, Wilimas J, Buckley PJ, Webber BL. Developmental cystic renal neoplasms in children. Diagnostic imaging characteristics. Comput Radiol. 1985 May-Jun;9(3):149-58.

1985 -- Pubmed # 4052944 -- Furman WL, Buckley PJ, Green AA, Stokes DC, Chien LT. Thymoma and myasthenia gravis in a 4-year-old child. Case report and review of the literature. Cancer. 1985 Dec 1;56(11):2703-6.

1985 -- Pubmed # 2982944 -- Buckley PJ, Dickson SA, Walker WS. Human splenic sinusoidal lining cells express antigens associated with monocytes, macrophages, endothelial cells, and T lymphocytes. J Immunol. 1985 Apr;134(4):2310-5.

1984 -- Pubmed # 6235743 -- Buckley PJ, Dickson SA. Monoclonal antibodies to T-helper/inducer and T-suppressor/cytotoxic lymphocyte subsets recognize antigens on splenic sinusoidal lining cells. Am J Clin Pathol. 1984 Aug;82(2):167-72.

1984 -- Pubmed # 6361149 -- Buckley PJ, Beelen RH, Burns J, Beard CM, Dickson SA, Walker WS. Isolation of human splenic macrophages and lymphocytes by countercurrent centrifugal elutriation. J Immunol Methods. 1984 Feb 10;66(2):201-17.

1984 -- Pubmed # 6421936 -- Walker WS, Beelen RH, Buckley PJ, Melvin SL, Yen SE. Some fixation reagents reduce or abolish the detectability of Ia-antigen and HLA-DR on cells. J Immunol Methods. 1984 Feb 24;67(1):89-99.

1981 -- Pubmed # 6270170 -- Leveston SA, McKeel DW Jr, Buckley PJ, Deschryver K, Greider MH, Jaffe BM, Daughaday WH. Acromegaly and Cushing's syndrome associated with a foregut carcinoid tumor. J Clin Endocrinol Metab. 1981 Oct;53(4):682-9.

1980 -- Pubmed # 6243769 -- Tanner DD, Buckley PJ, Hong R, Shearer WT. Fatal cytomegalovirus bronchiolitis in a patient with Nezelof's syndrome. Pediatrics. 1980 Jan;65(1):98-102.

1978 -- Pubmed # 659885 -- Buckley PJ, Wedner HJ. Measurement of the DNA synthetic capacity of activated lymphocytes: nucleotide triphosphate incorporation by permeabilized cells. J Immunol. 1978 Jun;120(6):1930-40.

1977 -- Pubmed # 301540 -- Buckley PJ, Wedner HJ. Variation in DNA and RNA synthetic responses during activation of lymphocytes from inbred strains of mice. J Immunol. 1977 Jul;119(1):9-18.

1973 -- Pubmed # 4579821 -- Howe CC, Buckley PJ, Carlson KM, Kozinski AW. Multiple and specific initiation of T4 DNA replication. J Virol. 1973 Jul;12(1):130-48.

1972 -- Pubmed # 4564205 -- Buckley PJ, Kosturko LD, Kozinski AW. In vivo production of an RNA-DNA copolymer after infection of Escherichia coli by bacteriophage T4. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3165-9.

1970 -- 1970. Miller RC, and Buckley PJ: Early intracellular events in the replication of bacteriophage T4 deoxyribonucleic acid. VI. Newly synthesized proteins in the T4-protein-deoxyribonucleic acid complex.  J. Virol. 5:502-6, 1970.

1969 -- Pubmed # 5778678 -- Krishnamurthi VS, Buckley PJ, Duerre JA. Pigment formation from L-tryptophan by a particulate fraction from an Achromobacter species. Arch Biochem Biophys. 1969 Mar;130(1):636-45.

1965 -- Pubmed # 5854590 -- Duerre JA, Buckley PJ. Pigment production from tryptophan by an Achromobacter species. J Bacteriol. 1965 Dec;90(6):1686-91.